10/99 WCCDG Meeting 
Modern HPLC Method Development & Optimization
Half-Day Workshop Presented by
Jeff Layne - Phenomenex Corp.
Thursday, October 21, 1999
8:30am - 12:00pm
Rm. 120, Plyler Hall
Furman University
Greenville, South Carolina
Agenda
| 8:15am-8:30am | Registration
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| 8:30am-12:00pm | Workshop
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| 12:00pm-1:30pm | Board Meeting
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Outline
- Part I. Column Selection - the right RP-HPLC column for a given application
- 1. Selectivity of phases - C18 vs Cyano vs Phenyl
- 2. Carbon Loading, Pore Size, Surface Area
- 3. Inertness
- 4. "Alternative" Phases
- Part II. Method Development - principles of basic method development
- 1. Use of mobile phase pH
- 2. Acetonitrile vs. Methanol
- 3. Use of ion-pair reagents
- 4. LC/MS Compatible mobile phase
- Part III. Optimizing sample through-put - maximizing productivity
- 1. Effects of changing flow rate
- 2. Effects of changing column length
- 3. Modifying gradient applications
The Speaker
Jeff Layne is a Senior Research Scientist at Phenomenex. Mr. Layne is a graduate of California State University Long Beach, where he received his Master of Science degree in Biology. Jeff has been with Phenomenex for over 2 years, starting out as a research assistant and quickly working his way up to senior research scientist, due to his in depth understanding of column chemistry. Jeff is an integral part of the Phenomenex research and development team. Areas of focus include interacting with end-users to determine chromatographic needs, working with product development and support, as well as developing seminars to aid in the understanding of new column technologies and method development.
Reservations
The cost of the workshop is $40, and seating is limited, so please register early. Mail you check or money order, along with attendee names, addresses, phone numbers & E-mail addresses to:
WCCDG
P.O. Box 1427
Taylors, SC 29687
For any questions, please call Kaye Castillo at (864) 597-4630, or E-mail her at castillokb@wofford.edu.
Directions
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